| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.154999 |
| Chromosome: | chromosome 3 |
| Location: | 8241887 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199600 | BES2 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCGCTCCAGCTCCCGGATGTTCGCCTC |
| Internal bar code: | TATGGCGGGCTCTTTCCTAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 350 |
| LEAP-Seq percent confirming: | 27.3032 |
| LEAP-Seq n confirming: | 1710 |
| LEAP-Seq n nonconfirming: | 4553 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATGTGTGATTTGGGCTG |
| Suggested primer 2: | CTCGTACTTGCGCATTTTCA |