Insertion junction: LMJ.RY0402.155030_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.155030
Chromosome:chromosome 2
Location:2143489
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g089274 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCTCGCACTCAAACACAACCACGCCCA
Internal bar code:GGACGACGGCGGGCGCCGAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:204
LEAP-Seq percent confirming:97.6003
LEAP-Seq n confirming:2481
LEAP-Seq n nonconfirming:61
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCGTAGGTGCATTGGTTA
Suggested primer 2:COULD_NOT_FIND_PRIMER