| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.155043 |
| Chromosome: | chromosome 3 |
| Location: | 6012570 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190900 | (1 of 5) PTHR10351:SF42 - LD40663P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACCTCAAGACCGACTTCAGGTGCGCCG |
| Internal bar code: | GCGCGATAAGGCGCGACGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 970 |
| LEAP-Seq percent confirming: | 99.7685 |
| LEAP-Seq n confirming: | 1293 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCAGAGCACCTGTTCA |
| Suggested primer 2: | TTTCACTACACCCGTTTCCC |