| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155064 |
| Chromosome: | chromosome 6 |
| Location: | 8203197 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305500 | (1 of 3) 3.5.1.110 - Peroxyureidoacrylate/ureidoacrylate amidohydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCACTGCACTTCGTGATGTTTGTTAGGG |
| Internal bar code: | GTGGTTCGATCCCTGCGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 92.0 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTATGCATCCCACACAG |
| Suggested primer 2: | CGCCTATTCTGCTGAGTTCC |