| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.155066 |
| Chromosome: | chromosome 2 |
| Location: | 4049174 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097227 | Found in Chlamydomonas and Volvox but not strongly conserved elsewhere | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGCGAGCTGGTTGCAGGACGCCTCAT |
| Internal bar code: | CTAGCTAGGACCGAGGATCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 340 |
| LEAP-Seq percent confirming: | 99.8296 |
| LEAP-Seq n confirming: | 586 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAGTTGCTACCCGTTGT |
| Suggested primer 2: | CGTTGTAAAAGAGCCCGGTA |