Insertion junction: LMJ.RY0402.155078_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.155078
Chromosome:chromosome 7
Location:1718977
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g325711 (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCACCGGCTGCAGGACTGCCAGCACTCG
Internal bar code:GCCAGGGGTTGTCAGAGGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:118
LEAP-Seq percent confirming:97.6744
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TACCTGTCGCACACACCATT
Suggested primer 2:GCTAAATGCTGCTCCTCACC