Insertion junction: LMJ.RY0402.155095_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.155095
Chromosome:chromosome_2
Location:312962
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g075350 CNK1 NimA-related protein kinase antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):ACCGTCCGAGGTCCAGCGGCGCGCCACTGG
Internal bar code:ACGACTTCCTTAGCATGTCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1
LEAP-Seq percent confirming:99.4413
LEAP-Seq n confirming:356
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGCTTTGGATCCTGTTGT
Suggested primer 2:CTCACCAACATCTGCCACAG