| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155095 |
| Chromosome: | chromosome 2 |
| Location: | 312962 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075350 | CNK1 | NimA-related protein kinase; (1 of 1) PTHR24361//PTHR24361:SF410//PTHR24362 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED // SERINE/THREONINE-PROTEIN KINASE NEK | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGTCCGAGGTCCAGCGGCGCGCCACTGG |
| Internal bar code: | ACGACTTCCTTAGCATGTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.4413 |
| LEAP-Seq n confirming: | 356 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCTTTGGATCCTGTTGT |
| Suggested primer 2: | CTCACCAACATCTGCCACAG |