| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155134 |
| Chromosome: | chromosome 16 |
| Location: | 3646536 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686641 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAATTCCGCAAGGATTACTGGCGCAGTG |
| Internal bar code: | AGAACGCGCGCCTCGGTAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 73.7121 |
| LEAP-Seq n confirming: | 14351 |
| LEAP-Seq n nonconfirming: | 5118 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAACGGTGAAATGCTGTG |
| Suggested primer 2: | AAGATCCTTTGCCTGCTGAA |