| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.155312 |
| Chromosome: | chromosome 9 |
| Location: | 1753135 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396500 | HAD3 | Halo-acid dehalogenase-like hydrolase; (1 of 98) IPR023214 - HAD-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGACGTATGGCCATACCGCGGTTCTCAA |
| Internal bar code: | CTCACTATGTGCCGGTCCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1215 |
| LEAP-Seq percent confirming: | 98.961 |
| LEAP-Seq n confirming: | 381 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGCGAAAGCCTCACAAG |
| Suggested primer 2: | ACCACCCACTCCACTCACTC |