| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.155440 |
| Chromosome: | chromosome 9 |
| Location: | 7940811 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g417400 | (1 of 1) PF00628//PF05970 - PHD-finger (PHD) // PIF1-like helicase (PIF1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCCAAGTTGCTCCTTGCGCAGTGCACA |
| Internal bar code: | ATACTGTTGGATTTTCGATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 98.4293 |
| LEAP-Seq n confirming: | 188 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAATGTAATGGACACGCAC |
| Suggested primer 2: | CGGGCTGTTAGCTCCTGTAG |