Insertion junction: LMJ.RY0402.155486_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.155486
Chromosome:chromosome_8
Location:2559912
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre08.g372500 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GAGGGGAGGGCAAGGAGGGAATGATGGTGA
Internal bar code:CAGTCACAGATAGTGCGGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:728
LEAP-Seq percent confirming:89.7651
LEAP-Seq n confirming:157746
LEAP-Seq n nonconfirming:17986
LEAP-Seq n unique pos:192

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCCCAAAGTAAACCAAA
Suggested primer 2:ACACTCGTCGCCCAAAATAC