| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.155522 |
| Chromosome: | chromosome 8 |
| Location: | 3993054 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379050 | (1 of 3) PF04438 - HIT zinc finger (zf-HIT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCGCATGGGAAAAGTAGGCTGAGCGCA |
| Internal bar code: | ACGCGGGGGACGGTCTCACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 901 |
| LEAP-Seq percent confirming: | 99.4115 |
| LEAP-Seq n confirming: | 3716 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGGTCGTGGTGCGTTTTA |
| Suggested primer 2: | GTCGAGTGTGAGACGCGATA |