| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155524 |
| Chromosome: | chromosome_1 |
| Location: | 3820000 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g024750 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATGGACCCATATAGCCCTGAGCCCCTAAC |
| Internal bar code: | GGGTCGCAGGCCCGCTACACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 93.0693 |
| LEAP-Seq n confirming: | 564 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTGGACGTCCTTTCCTG |
| Suggested primer 2: | GTTGCCCGTCATCTTACGTT |