| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.155527 |
| Chromosome: | chromosome 15 |
| Location: | 1089915 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638304 | NCL30,OPR74 | Nuclear Control of chloroplast Like 30 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCGGACAACGAGGATGCGTCTTTTCTG |
| Internal bar code: | GCACTCGGTACCGTGAAATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 99.1597 |
| LEAP-Seq n confirming: | 1534 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATTGAACACCAGCAGAC |
| Suggested primer 2: | GTAATTGATGCGGACGGACT |