| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155565 |
| Chromosome: | chromosome 3 |
| Location: | 3579933 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168250 | (1 of 6) PF06701 - Mib_herc2 (MIB_HERC2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGGTCCCTGCTGCTTTGGATTTGGTTT |
| Internal bar code: | GCCAACTCCCCGGAGTAGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 63.1579 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATCAAATGCTGGGCTGTC |
| Suggested primer 2: | CAAGCAACCTATGACTCGCA |