| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.155568 |
| Chromosome: | chromosome 3 |
| Location: | 4284058 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174500 | HDZ1 | (1 of 1) PF00046 - Homeobox domain (Homeobox); Zygote-specific homeobox protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTCCTGTTTAATCGGGCTGTGGCGGTC |
| Internal bar code: | CGTGGGCACGCAGCGCAAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 169 |
| LEAP-Seq percent confirming: | 58.165 |
| LEAP-Seq n confirming: | 11031 |
| LEAP-Seq n nonconfirming: | 7934 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTGACAGCTTGTCTGGA |
| Suggested primer 2: | ATGCTGATTGACTGCACGAG |