| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.155568 |
| Chromosome: | chromosome 17 |
| Location: | 3383030 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723850 | (1 of 1) PF06775 - Putative adipose-regulatory protein (Seipin) (Seipin) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTAGGAGGTTTCAACGGCGTAACAAAAA |
| Internal bar code: | AGGTATTGGGCAAATGCTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1152 |
| LEAP-Seq percent confirming: | 99.5878 |
| LEAP-Seq n confirming: | 4107 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGATGTCAACTTACGCC |
| Suggested primer 2: | CTATTGCGCCTCCAAGTAGC |