| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.155572 |
| Chromosome: | chromosome 9 |
| Location: | 4127930 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393728 | (1 of 3) K05302 - SET domain-containing protein 6 (SETD6) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGCAGAGCCGCGCCTTCCACCTGGAG |
| Internal bar code: | CGTTTTATCCCGTTTTATGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 99.6112 |
| LEAP-Seq n confirming: | 1281 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGACAAAAACAAGGGCAC |
| Suggested primer 2: | GAAGAAGCTTTTGATTGCGG |