| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.155595 |
| Chromosome: | chromosome 12 |
| Location: | 1664654 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486050 | (1 of 1) PF00098//PF00397 - Zinc knuckle (zf-CCHC) // WW domain (WW) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAAAACGGCGCCAGTGGCCTTGGTCTG |
| Internal bar code: | TACGCATGCGGGCTACTGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 425 |
| LEAP-Seq percent confirming: | 74.597 |
| LEAP-Seq n confirming: | 1157 |
| LEAP-Seq n nonconfirming: | 394 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGCCAAGCTTAACAGC |
| Suggested primer 2: | CACTGTGAAAGACTTCGGCA |