| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155619 |
| Chromosome: | chromosome 6 |
| Location: | 4200332 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278234 | FAP343 | (1 of 19) PF00397 - WW domain (WW); Flagellar Associated Protein 343 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGTGCCTCGTGGGCTGCAGGGGACAT |
| Internal bar code: | GGAAATGATGAAGTGTCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 89 |
| LEAP-Seq percent confirming: | 90.1709 |
| LEAP-Seq n confirming: | 211 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGTGTTTCTGTGTGGGGA |
| Suggested primer 2: | TCCGAGACATGTACGAGCAG |