| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.155625 |
| Chromosome: | chromosome 16 |
| Location: | 1420612 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652350 | CMA1,FAP183 | Chlamydomonas-specific membrane protein family A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACACTAGTTGTTATGGTGGAATACTGGA |
| Internal bar code: | CCGGTACATGCACTGGCGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 99.6154 |
| LEAP-Seq n confirming: | 259 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCATGAGGCAGCTGAAC |
| Suggested primer 2: | CGATTGACGTGATTGGAATG |