| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155664 |
| Chromosome: | chromosome 16 |
| Location: | 3170631 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666100 | (1 of 1) K14168 - cytoplasmic tRNA 2-thiolation protein 1 (CTU1, NCS6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCCTGTTCAGCGGTGGCCATGGCGGTG |
| Internal bar code: | GGATCGTGTCGTCCGAACGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1047 |
| LEAP-Seq percent confirming: | 99.3496 |
| LEAP-Seq n confirming: | 1222 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAATCAGGGCGGATCTTCA |
| Suggested primer 2: | TGCTGTGCCTGCAGATTTAC |