| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.155665 |
| Chromosome: | chromosome 14 |
| Location: | 3683278 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631600 | (1 of 1) 1.5.1.40 - 8-hydroxy-5-deazaflavin:NADPH oxidoreductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGGGCTTGGGGTAGTCGCCGACCAGC |
| Internal bar code: | TGTAACAACGCCGTTGACCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 140 |
| LEAP-Seq percent confirming: | 64.0625 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGGACGACTTTGGTTCG |
| Suggested primer 2: | CGAAACCGTGGGTTATATGG |