| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.155692 |
| Chromosome: | chromosome 12 |
| Location: | 6470092 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538250 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCTGAACACGCCGCGCACAGGCTGCTG |
| Internal bar code: | GCAACTGATGTGAGGGCGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 351 |
| LEAP-Seq percent confirming: | 99.5786 |
| LEAP-Seq n confirming: | 9688 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACACTGACAGCACCAGT |
| Suggested primer 2: | TCCGTGGCTTTCTACAGCTT |