Insertion junction: LMJ.RY0402.155694_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.155694
Chromosome:chromosome 12
Location:8862152
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g544750 (1 of 1) PF13637//PF13857 - Ankyrin repeats (many copies) (Ank_4) // Ankyrin repeats (many copies) (Ank_5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAAAGAAGTGAAGGGCGGGCCCTACTAG
Internal bar code:CGGTCGCGCGGCGTGTTCCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:427
LEAP-Seq percent confirming:96.679
LEAP-Seq n confirming:786
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GTACTTGCCATTTGACCGCT
Suggested primer 2:TACCTTGGCTCTCAGGCTGT