| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.155717 |
| Chromosome: | chromosome 12 |
| Location: | 1532500 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACACGGCTGCACGGGATGGCGTTGCAAT |
| Internal bar code: | TGCTTGCCGACGAAATGGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1080 |
| LEAP-Seq percent confirming: | 99.6086 |
| LEAP-Seq n confirming: | 12469 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCCACGAAACTATGCAG |
| Suggested primer 2: | TGGGTCTTAAATAGCAGCGG |