| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155734 |
| Chromosome: | chromosome_6 |
| Location: | 7757407 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g302200 | EFH3 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACAACCCGGGACAACAGGGACGCGACGAC |
| Internal bar code: | CCCTCACAGACATCCTGCACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 418 |
| LEAP-Seq percent confirming: | 40.4412 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATTCCTTTTGCCATGTCT |
| Suggested primer 2: | CATGATGGTAGGTGCTGGTG |