Insertion junction: LMJ.RY0402.155758_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.155758
Chromosome:chromosome 6
Location:8873904
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g310850 (1 of 12) IPR003439//IPR003593//IPR027417 - ABC transporter-like // AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCTCGCTCACCCTCGCTCACAGGGCTGA
Internal bar code:CCTCCCTCGGACGGGTAGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:468
LEAP-Seq percent confirming:99.8731
LEAP-Seq n confirming:787
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AAATCAAGGCCTAGCAAGCA
Suggested primer 2:CTTGCCATCAACACGCATAC