| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.155763 |
| Chromosome: | chromosome 17 |
| Location: | 4438543 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732050 | AGE3 | DNA repair glycosylase; (1 of 1) K10773 - endonuclease III (NTH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCAGGAGGCTGTACATACGGTACCAGG |
| Internal bar code: | AGAGAGGTGGGAGTATCTAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 99.931 |
| LEAP-Seq n confirming: | 1448 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTGTGAGTTGCTGAGCG |
| Suggested primer 2: | CTGACGATAGGAACGGAAGC |