Insertion junction: LMJ.RY0402.155765_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.155765
Chromosome:chromosome 17
Location:4174615
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g730000 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCATCCTGACCTGCACCGTGGGCACCCTG
Internal bar code:CACTACACGGATGCGGGTCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:368
LEAP-Seq percent confirming:71.167
LEAP-Seq n confirming:19667
LEAP-Seq n nonconfirming:7968
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTGTGTGTGTGTGGT
Suggested primer 2:TATGTGTTTGTGTGTGGGGG