| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.155771 |
| Chromosome: | chromosome_12 |
| Location: | 5559719 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g531500 | DRC3,FAP134 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGTACCGGCACCGCCCGCTTCAGCTGCCA |
| Internal bar code: | CTTTCTAGGGTACCGAGAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 77.3472 |
| LEAP-Seq n confirming: | 519 |
| LEAP-Seq n nonconfirming: | 152 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCAAGAGAACAGCGACA |
| Suggested primer 2: | TTTCCCACTCCTCCTCCTTT |