| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155791 |
| Chromosome: | chromosome 17 |
| Location: | 4023713 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCACAAAGGGTCACACCCACGCTACGG |
| Internal bar code: | AGCTATCAGCAGGCGCAAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 68.0723 |
| LEAP-Seq n confirming: | 113 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACAACCCTCCATCCCTCC |
| Suggested primer 2: | TAGCTGTGGCTAATGATGCG |