Insertion junction: LMJ.RY0402.155801_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.155801
Chromosome:chromosome 8
Location:3649549
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g376450 CPLD36 Conserved in the Plant Lineage and Diatoms; (1 of 1) PF12159 - Protein of unknown function (DUF3593) (DUF3593) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACTGGATACGGTGAGGCACTTCGGCTAC
Internal bar code:ACCCGTGATCCACGACGCTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:274
LEAP-Seq percent confirming:74.7748
LEAP-Seq n confirming:166
LEAP-Seq n nonconfirming:56
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAACTAAACTGAACCCGA
Suggested primer 2:TCCTATTCTGCCACTCCCAC