| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.155808 |
| Chromosome: | chromosome 3 |
| Location: | 1801117 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154250 | MAPKKK6 | (1 of 1) IPR000719//IPR001245//IPR002290//IPR011009//IPR013320//IPR020635 - Protein kinase domain // Serine-threonine/tyrosine-protein kinase catalytic domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain // Concanavalin A-like lectin/glucanase domain // Tyrosine-protein kinase, catalytic domain; Mitogen-Activated Protein Kinase Kinase Kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTATCACAGAACCCACATCCTGTGTCTGC |
| Internal bar code: | CTGCGACACCTGGCGGTAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.3475 |
| LEAP-Seq n confirming: | 609 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACGTCAACTCTCCTCGG |
| Suggested primer 2: | ACTGTTGGAGCATTGCTGTG |