| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.155809 |
| Chromosome: | chromosome 11 |
| Location: | 237120 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467554 | (1 of 15) PF01694 - Rhomboid family (Rhomboid) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCACGTACACGTACAGAGGCCGGACCTG |
| Internal bar code: | TTGGGACGATGGAAGTGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAATCTCCATCCCTCCACG |
| Suggested primer 2: | ACCTGCCACAACTATCCCTG |