| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155810 |
| Chromosome: | chromosome 3 |
| Location: | 5735207 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188100 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACCACCACCGCCACCACCACCCGCTCC |
| Internal bar code: | ATCGTCCGGCTTGGCTGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 42.3804 |
| LEAP-Seq n confirming: | 381 |
| LEAP-Seq n nonconfirming: | 518 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTGGTGGTTGTGTGAAG |
| Suggested primer 2: | GGTCACTTTGAGACTTCGGC |