| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155839 |
| Chromosome: | chromosome 9 |
| Location: | 4998999 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398030 | (1 of 1) PF07744 - SPOC domain (SPOC) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAGTGCAGAGACTTCAGACACTACAGA |
| Internal bar code: | GCGGGGCTCCGAGTGTGTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 99.7743 |
| LEAP-Seq n confirming: | 1768 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCTGCGACACCGTACCT |
| Suggested primer 2: | CCCTTAGTGGCGTAGAGCTG |