Insertion junction: LMJ.RY0402.155841_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.155841
Chromosome:chromosome 16
Location:2500130
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g660900 KIL10 Putative kinesin-like motor protein; (1 of 1) PTHR24115//PTHR24115:SF0 - FAMILY NOT NAMED // KINESIN-LIKE PROTEIN KLP10A intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCTGGTATGTACAACCCCCACCGGAGC
Internal bar code:TACTCTTGCTCAATGTGTCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:396
LEAP-Seq percent confirming:7.70713
LEAP-Seq n confirming:120
LEAP-Seq n nonconfirming:1437
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTCACCTTTTCTTTGAG
Suggested primer 2:CCACTGTCACCCACTGTCAC