| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155841 |
| Chromosome: | chromosome 17 |
| Location: | 1727028 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708901 | (1 of 20) PF01822 - WSC domain (WSC) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTAGTACGACTGGTCTGGGCCGATCACA |
| Internal bar code: | GGGACAGATATGCTCCTCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 69.1235 |
| LEAP-Seq n confirming: | 347 |
| LEAP-Seq n nonconfirming: | 155 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGACCGTATGCTCCAAC |
| Suggested primer 2: | AAACACACACGCGCTACTTG |