| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155850 |
| Chromosome: | chromosome 5 |
| Location: | 2232049 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234658 | (1 of 1) PTHR13480:SF0 - E3 UBIQUITIN-PROTEIN LIGASE HAKAI | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGACCCCATGTCCGTGGCACATCCCAG |
| Internal bar code: | TCGTGCGGGTGACGTAAAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1058 |
| LEAP-Seq percent confirming: | 99.6067 |
| LEAP-Seq n confirming: | 1013 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCAGAGCCACAAAAGGTG |
| Suggested primer 2: | GTTGGGCTTCTCCACACATT |