Insertion junction: LMJ.RY0402.155857_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.155857
Chromosome:chromosome 12
Location:7260078
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g558100 PRMT2,PRMT10,PRM2 Protein-/Histone-arginine N-methyltransferase; (1 of 1) PTHR11006:SF68 - PROTEIN ARGININE N-METHYLTRANSFERASE PRMT10 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGCTCGCTGCGGTAGCCGTCAGACAGGA
Internal bar code:GTAGCTCGCGGGTTCTTAGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:835
LEAP-Seq percent confirming:99.7729
LEAP-Seq n confirming:4393
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGTCTGTGGGGTTCTCGT
Suggested primer 2:GAGGTTGGAGAGGAGCTGTG