| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155871 |
| Chromosome: | chromosome 17 |
| Location: | 3476491 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724950 | (1 of 1) PTHR16161:SF0 - TRANSCRIPTIONAL PROTEIN SWT1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCGCTCCTTGCAAAGCGGTCTAGCTTT |
| Internal bar code: | GCGCGCAGGTAAGCCTGACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 98.9841 |
| LEAP-Seq n confirming: | 2923 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTCAAGTATGAACCCGT |
| Suggested primer 2: | TCTGTCTCGTGCATCTAGCG |