| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.155878 |
| Chromosome: | chromosome 12 |
| Location: | 2121520 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509000 | MAPK3 | Mitogen-Activated Protein Kinase 3; (1 of 3) K04371 - mitogen-activated protein kinase 1/3 (MAPK1_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGATCAAGCTCCTCCGGCACTTGAAAC |
| Internal bar code: | CTCAAGGTGATTCAGGCCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 99.7328 |
| LEAP-Seq n confirming: | 4479 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCGGTGCCCTAATAAAC |
| Suggested primer 2: | GACCAGAAATTGGCAACGAT |