| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155882 |
| Chromosome: | chromosome 7 |
| Location: | 3771863 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338250 | (1 of 4) PF04073 - Aminoacyl-tRNA editing domain (tRNA_edit) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAATCAGTCTGGGCACCCTTGGTGTTGTC |
| Internal bar code: | CTGTCCTGCGGTAGACCGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 99.7615 |
| LEAP-Seq n confirming: | 1673 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGTATCGCGTGGCCTTAC |
| Suggested primer 2: | ATCATGGTGTGAACAGGGGT |