| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.155883 |
| Chromosome: | chromosome 10 |
| Location: | 6324879 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465300 | (1 of 89) 3.6.4.13 - RNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCTTCCCCTCGGCCGCGGCCTTCGGGC |
| Internal bar code: | GCGCGGACTACAACGTAGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 63.75 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAACTGGGTGAAGTGGGT |
| Suggested primer 2: | TCTCGCACAGCTTAAAGGGT |