| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.155890 |
| Chromosome: | chromosome 7 |
| Location: | 1031220 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319500 | (1 of 2) 2.3.2.15 - Glutathione gamma-glutamylcysteinyltransferase / Phytochelatin synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCGCGCCGTTGCAGCGGGCCAGGCAGG |
| Internal bar code: | GTATGCCAGTAGACTTCGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 65.587 |
| LEAP-Seq n confirming: | 162 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAGGACACGTGAGGTTT |
| Suggested primer 2: | GGAGTAGGTCCCCATACGGT |