| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.155909 |
| Chromosome: | chromosome 2 |
| Location: | 4004174 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097000 | DHP1 | (1 of 1) K01464 - dihydropyrimidinase (DPYS, dht, hydA); Dihydropyrimidinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGCCGGGGTTCGGCCACCCTCTCACTC |
| Internal bar code: | GGGAGGTACAACGACGTACGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 35 |
| LEAP-Seq percent confirming: | 99.0099 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGAACCATGACCAGCAGT |
| Suggested primer 2: | GGAACGGGTGCAAGAGATAA |