| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.155929 |
| Chromosome: | chromosome 9 |
| Location: | 5273077 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399701 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGATTGCACATAACCAACTGCACCACAG |
| Internal bar code: | GTATGGGACACGTCGCGTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 95.9596 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTAAACCTAAGAACGGCG |
| Suggested primer 2: | TCAGCACGTTGGTCTGACTC |