| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.155930 |
| Chromosome: | chromosome 17 |
| Location: | 1249188 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705250 | (1 of 3) IPR002889//IPR013994 - Carbohydrate-binding WSC // Carbohydrate-binding WSC, subgroup | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCAAGCGGCGGCAACAACACGGGCGTG |
| Internal bar code: | GAAAGTATTATGTTTTTACTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1123 |
| LEAP-Seq percent confirming: | 99.4956 |
| LEAP-Seq n confirming: | 3156 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAATACCTGGCGAGAATC |
| Suggested primer 2: | CAACAGTAGCCTGACCAGCA |