| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.156004 |
| Chromosome: | chromosome 6 |
| Location: | 3746292 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278153 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCGCCCTTCCCGCTCTCAACATCCACC |
| Internal bar code: | CTGCACCGTCAGTCATAGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 929 |
| LEAP-Seq percent confirming: | 93.5151 |
| LEAP-Seq n confirming: | 6619 |
| LEAP-Seq n nonconfirming: | 459 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTGTATGTGGTGTGTTG |
| Suggested primer 2: | CTAAGTACCGCACTGCCCTC |